1. In this article, using Gateway technology GHF gene expression vector was constructed and introduced into Arabidopsis. It was useful for further gene functional research.
2. In the algorithm, the conditional linear state equation is first inserted into the measurement equation, which fuses the linear state process noise and the original measurement noise, whereafter the GHF is used to estimate the nonlinear states. Then the estimated means of the nonlinear states are inserted into the linear state equation and the original measurement equation to estimate the linear states by the KF. Moreover, in order to improve the accuracy of the estimates, the estimated variances of the nonlinear states are fed back to modify the estimations of the linear states using the KF.